PURPOSE: To provide a procedure to operate and maintain the Shimadzu make Class VP series Gradient HPLC system. SCOPE: This procedure is applicable for the GRADIENT HPLC, Shimadzu -Class VP series used in the Quality Control department III. RESPONSIBILITY: It is the responsibility of all the personnel involved in the analysis using of the system IV. DOCUMENT REFERENCE: SOPs: Core procedure: Calibration of Equipment Forms: Calibration of HPLC V. OPERATION: 1.0 PRELIMINARY CHECK: 1.1 Check that the instrument is clean and free from dust, if not, clean with a soft cloth duster. 2.0 THE LC-10 AVP SERIES HPLC WORK STATION CONSISTS OF: 2.1. LC-10AT VP Pump 2.2.

Choose the most popular programs from Communication software. Please visit the main page of Class-VP - CClass-VP on Software Informer. Shimadzu Corporation. The effects of cadmium chloride on secondary metabolite production in Vitis vinifera The compounds were quantified using Shimadzu CLASS-VP software..

SPD-10 AVP UV vis detector 2.3. SIL-HT- A System controller and auto sampler 2.4. Computer with Class VP software version 6.10 3.0 BASIC OPERATION: 3.1.

Switch ‘ON’ the main for LC-10 pump 3.2. Switch ‘ON’ the main for auto injector 3.3.

Switch ‘ON’ the main for UV vis detector 3.4. Switch ‘ON’ the system controller and the computer. After switching ‘ON’ all the components, finally switch ‘ON’ the computer. Default screen appears on the respective screen modules. Double click on the (Shimadzu class VP) icon in the screen. Double click on Instrument 1 icon. Here for the beep sound, which indicates the communication between the system and the software.

OPERATING PROCEDURE FOR LC-10AVP PUMP 3.10. Open the drain valve of the pump. Press the purge key. Ensure that there are no air bubbles in the flow line and press purge/pump key to stop purging. Close the drain valve of the pump.

SYSTEM SOFTWARE 3. Elettrotecnica Ed Elettronica Pdf File. 11 Check the method name displayed on the top of the screen. 3.12 Click on the method option, click the instrument set up.

Click on the pump, set the A. Flow in ml/min. P.max in kgf/cm2. 3.13 Click on the detector SPD-10 AVP, icon. Set the wavelength in nm and run time in min. and save the method. 3.14 After setting the above parameters, click on download at the bottom of the screen.

3.15 Observe for the message displayed on the screen. Click the OK icon and close the screen. This indicates the software successfully downloaded the set information to the system. 3.16 Click on sequence. Click on edit, edit table will appear.